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Pooja Singh¹, Claudeen Sze Siang Lau¹, Sze Yin Siah¹

¹School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500, Bandar Sunway, Selangor, Malaysia.

Archives of microbiology

|March 23, 2024

概括

四种细菌分离物降解了多种塑料,如聚乙烯和聚烯. 这些微生物产生酶,将聚合物分解成更小的分子,为塑料污染提供可持续的解决方案.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
聚合物科学聚合物科学

背景情况:

塑料污染是一个重大的全球环境挑战.
生物降解为塑料废物管理提供了一种可持续的方法.
像聚乙烯 (PE),聚乙烯二甲 (PET) 和聚烯 (PS) 这样的反性聚合物是持久性污染物.

研究的目的:

选和识别能够降解多种类型的回收性塑料的细菌分离物.
为了研究参与聚合物生物降解的酶活性 (乳酶和酶).
描述聚合物的降解产品和表面变化.

主要方法:

从塑料污染环境中分离细菌 (红树林和垃圾填埋场).
用低密度聚乙烯 (LDPE),聚乙烯二甲 (PET) 和聚烯 (PS) 化细菌分离物4周.
酶活性测定 (乳酶,雌酶).
使用富里埃变换红外光谱法 (FTIR),液态染色学质谱法 (LC-MS) 和场辐射扫描电子显微镜 (FESEM) 进行分析.

主要成果:

有四种细菌分离物 (Dermacoccus sp. 在 MR5 中,Corynebacterium sp. 在MR10中,Bacillus sp. BS5,普里斯蒂亚 (Priestia) 种类. TL1) 证明了降解能力.
巴西勒斯种类 (Bacillus sp.) 的存在在BS5中,乳糖酶的产量最高 (15.35U/mL),并且在PS中引起显著的体重减轻 (4.84%).
FTIR和LC-MS证实了聚合物的氧化和分解成更小的分子.
在聚合物样本上,FESEM揭示了细菌殖民,生物膜形成和表面侵蚀.

结论:

鉴定到的细菌分离物具有能够降解各种反抗性聚合物的酶机制.
这些微生物显示出塑料污染环境的生物修复潜力.
进一步的研究可以优化各种塑料类型增强生物降解的条件.

关键词:

酶是一种酶.场辐射扫描电子显微镜里埃变换红外光谱法红外光谱法液体染色学质谱学质谱学红树林是一种红树林.卫生垃圾填埋场卫生垃圾填埋场

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