种子传播的细菌合成社区抵抗种子致病菌并促进植物生长
De-Lin Luo1, Shi-Yi Huang1, Chen-Yu Ma1
1Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology and Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China.
Journal of applied microbiology
|March 23, 2024
概括
合成微生物群落有效地保护花生种子免受阿斯伯吉路斯黄菌的 aflatoxin污染,并防止由Fusarium oxysporum引起的根腐烂,促进整体植物生长.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物技术是生物技术.
背景情况:
- 种子受到Aspergillus flavus的污染,导致亚毒素的产生,对作物产量和安全性构成重大威胁.
- Fusarium oxysporum会导致各种植物物种的破坏性根腐,影响农业生产率.
- 开发可持续的策略来控制这些病原体对于粮食安全至关重要.
研究的目的:
- 评估来自花生种子细菌的合成微生物群落 (SMC) 在控制亚毒素污染和由Fusarium oxysporum诱导的根腐烂方面的有效性.
- 评估SMC对花生幼苗生长和防御机制的影响.
主要方法:
- 建设三个不同的SMC:保存 (C),促进增长 (S) 和联合 (CS).
- 利用16S rRNA Illumina测序用于社区分析,菌株隔离和植物生长促进试验.
- 用SMC注射花生苗并评估它们对F. oxysporum和A. flavus的耐药性,以及防御酶活性和酸 (SA) 路径激活.
主要成果:
- 这三种SMC都表现出对根腐蚀的抵抗力,联合社区 (CS) 显示出最显著的效果.
- 在花生幼苗中,CS接种导致防御酶活性增加,并激活与SA相关的全身获得抵抗.
- 该CS社区有效地抑制了A. flavus在种子上的繁殖,并减少了亚毒素的产生,这归因于细菌毒素降解和菌根破坏.
结论:
- 合成的花生种子细菌群体可以有效地用于保护花生免受A. flavus种子入侵和F. oxysporum幼苗感染.
- 这些SMC的应用促进了花生幼苗的生长,并增强了植物对病原体的防御机制.
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