可光疗的细胞外基质密封剂用于停止静脉出血
Luke E Schepers1, Brooke L Martindale2, Alycia G Berman2
1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
概括
一种新的可光修复的细胞外基质密封剂 (pcECM) 能够有效地阻止静脉出血并随着时间的推移降解. 这种耐用密封剂提供可调节的机械性能,用于手术应用和血液控制.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 手术创新 在外科创新.
背景情况:
- 出血是导致死亡的主要原因,需要有效的长期出血控制,超越初始压力或带.
- 目前的静血剂需要特殊的储存和制备,限制了它们在各种环境中的使用.
- 可光固化细胞外基质 (pcECM) 密封剂提供了潜在的优势,如室温储存和快速准备.
研究的目的:
- 评估一种用于控制静脉出血的新型可光愈细胞外矩阵密封剂 (pcECM).
- 为了比较pcECM的疗效,体内粘附和降解特征与商用纤维素密封剂 (TISSEEL®) 相比.
- 评估pcECM交联密度对其机械性能和生物反应的影响.
主要方法:
- 用一种小鼠模型来评估pcECM阻止静脉出血的能力.
- 根据pcECM交联密度分析了机械性能 (粘度,膨胀,破裂强度,抗拉强度,弹性).
- 在体内降解,伤口粘附和局部血液动力学/炎症反应在28天内使用3D超声波和探索分析进行了监测.
主要成果:
- pcECM配方表现出受交联密度影响的可调节的机械性能.
- 在所有测试的案例中,pcECM和TISSEEL®都成功地封闭了静脉缺陷.
- 到28日,pcECM的降解率降至17±8%的初始体积,在两种密封剂中观察到暂时的局部血液动力学变化和急性炎症.
结论:
- pcECM是一种耐用密封剂,具有可调节的机械特性,适合控制出血.
- 密封剂有效地密封容器,并在14-28天的时间内降解.
- pcECM为手术静血提供了一个有希望的替代方案,特别是在需要方便的储存和准备的环境中.
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