人类肠道肠体平台评估废水中胃肠炎病毒的传染性
Noelia Carmona-Vicente1, Annamaria Pandiscia2, Cristina Santiso-Bellón1
1Department of Microbiology and Ecology, University of Valencia, Valencia, Spain.
Water research
|March 23, 2024
概括
人类诺罗病毒和其他胃肠炎病毒在废水中现在可以使用一种新型的人类肠道肠杆菌模型来检测. 这一突破允许更好地评估与污染水源相关的病毒感染性和公共卫生风险.
科学领域:
- 病毒学 病毒学
- 公共卫生 公共卫生
- 环境科学 环境科学
背景情况:
- 便-口腔传染性胃肠炎病毒,特别是人类诺罗病毒 (HuNoVs),对公共健康构成重大风险.
- 在受污染的水中评估病毒感染性是具有挑战性的,因为在细胞系中培养和测量病毒感染性的困难.
- 人类肠道肠 (HIE) 为研究肠道病毒复制提供了一个有前途的模型.
研究的目的:
- 评估HIE模型在废水中检测多种胃肠炎病毒感染的可行性.
- 在HIE模型中优化病毒复制条件.
- 使用HIE模型从废水样本中证明自然存在的胃肠炎病毒的传染性.
主要方法:
- 通过评估影响病毒复制的因素来优化HIE模型,利用3D HIE的试剂辅助破坏.
- 人类诺罗病毒 (HuNoV) GI和GII,轮状病毒 (RV),天体病毒 (HAstV) 和腺病毒 (HAdV) 在单一和并发感染中的复制率.
- 将优化的HIE模型应用于28个废水样本,然后进行 (RT) -qPCR分析.
主要成果:
- 在HIE模型中,多种胃肠炎病毒的复制产量具有可比性.
- 28个废水样本中有16个 (57%) 显示了HuNoVs,RV,HAstV和/或HAdV的复制.
- 三个样本证实了HuNoV与特定基因型的复制,四个样本显示了多个病毒的同时复制.
结论:
- 人体肠道肠杆菌体 (HIE) 模型有效地证明了废水中的多种人类胃肠炎病毒的传染性.
- 该模型有助于在环境样本中检测传染性病毒,包括人类诺罗病毒.
- 这些发现有助于更好地了解污染水的病毒传播风险.
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