MYB转录因子SmMYB113直接调节茄子中依赖乙烯的花脱落
Jing Chen1, Senlin Jiang1, Guobin Yang1
1College of Horticulture Science and Engineering, Shandong Agricultural University, Shandong, 271018, China.
Plant physiology and biochemistry : PPB
|March 23, 2024
概括
转录因子SmMYB113直接触发茄子花的乙烯生产,导致花下降的增加. 这项研究揭示了SmMYB113用于调节依赖乙烯的花脱落的分子通路.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 园艺科学 园艺科学
背景情况:
- 花的脱落会对园艺作物的产量产生负面影响.
- 此前,SmMYB113已被确定为改善水果质量的因素,但导致茄子中花落的增加.
研究的目的:
- 为了阐明SmMYB113在茄子花脱落中的调节机制.
- 为了研究乙烯生物合成和信号在SmMYB113介导的花落中的作用.
主要方法:
- RNA测序 (RNA-seq) 用于识别差异表达的基因.
- 乙烯含量分析和以乙烯 (ETH) 和1-甲基cyclopropene (1-MCP) 的处理.
- 酵母一混合,双化酶试验,酵母两混合 (Y2H),双分子光补充 (BiFC) 和化酶补充试验,以验证分子相互作用和基因调节.
主要成果:
- SmMYB113过度表达改变了乙烯生物合成基因 (SmACS1,SmACS8,SmACO4) 的表达.
- SmMYB113 直接调节依赖乙烯的花脱落.
- SmMYB113与SmACS1,SmACS8和SmACO4.4的促进体结合并激活它们.
- SmERF38与SmMYB113相互作用,并增强其对SmACS1促进剂活性的影响.
结论:
- SmMYB113通过直接调节乙烯生物合成基因,促进茄子中的花.
- 在这个过程中,SmMYB113的功能得到增强,SmERF38起到积极的调节作用.
- 这项研究揭示了一种新的分子机制,它是茄子中依赖乙烯的花脱落的基础.
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