以异构体为导向的45种血胆酸的分离,用液态染色学-并联质谱法进行分离
Lei Zhang1, Xu Liu2, Tenghui Jin3
1Center of Clinical Medical Research, Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing 100191, China; Drug Clinical Trial Center, Peking University Third Hospital, Beijing 100191, China.
Journal of chromatography. A
|March 23, 2024
概括
这项研究开发了一种敏感的LC-MS/MS方法来量化45个胆酸 (BA),克服异构体的挑战. 该方法揭示了BA和葡萄糖/胰岛素之间的相关性,突出了它们作为代谢障碍生物标志物的潜力.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
背景情况:
- 胆汁酸 (BAs) 被认为是代谢疾病的生物标志物和治疗点.
- 由于同位素和灵敏度限制,同时量化多个BA具有挑战性.
研究的目的:
- 开发和验证一种敏感的LC-MS/MS方法,用于同时量化具有相似极性的多个胆酸.
- 应用该方法来分析高葡萄糖摄入量后BA的动态变化,并探索它们作为代谢生物标志物的作用.
主要方法:
- 使用九个稳定同位素内部标准,开发和验证一种液态染色学-并联质谱法 (LC-MS/MS) 方法,用于同时量化45种胆酸.
- 优化色谱和质谱条件,以实现以同位素为导向的分离和高灵敏度.
- 应用验证的方法来分析高葡萄糖挑战后健康个体的血样本.
主要成果:
- 建立了一个强大的LC-MS/MS方法,量化45个胆酸,其下限量化范围在0.05-10 ng/mL之间.
- 该方法表现出极好的线性,准确性,精度和稳定性.
- 在血样本中量化了35种胆酸,显示高葡萄糖摄入后的动态变化.
- 在胆汁酸,葡萄糖和胰岛素水平之间观察到显著的相关性.
结论:
- 与以前的研究相比,开发的LC-MS/MS方法为同时胆酸量化提供了更高的灵敏度和分离效率.
- 胆酸显示出作为代谢障碍的有价值生物标志物的潜力,其与葡萄糖和胰岛素的相关性证明了这一点.
- 这种方法为进一步研究胆酸在代谢健康和疾病中的作用提供了一个强大的工具.
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