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原蛋白PHD指转录因子 (BPTF) 对热囊细胞上皮质转介质转变的作用

Linqing Pan¹, Fuquan Zhu², Aochen Yu²

¹College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Clinical Center of Reproductive Medicine, Lianyungang Maternal and Child Health Hospital, Kangda College of Nanjing Medical University, Lianyungang 222000, China.

|March 23, 2024

概括

原体PHD指转录因子 (BPTF) 缺陷会损害热原体上皮质到介质酶过渡 (EMT),这是怀孕的关键过程. 降低的BPTF阻碍了热囊细胞的入侵,并与反复流产 (RM) 有关.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

热原体上皮质转移到介质细胞转移 (EMT) 对成功怀孕至关重要,包括胚胎植入和胎儿与母亲的沟通.
不足的热囊细胞EMT与重复性流产 (RM) 的病理有关.
整体外体序列测定确定了与RM相关的原体PHD指转录因子 (BPTF) 的突变.

研究的目的:

调查BPTF在 trofhoblast EMT中的作用.
阐明BPTF影响EMT的潜在分子机制.
为了确定BPTF表达和反复流产之间的关联.

主要方法:

从RM患者的小体中分析BPTF表达.
基因本体学 (GO) 分析以确定与BPTF相关的功能.
试验室内实验涉及BPTF敲击,以评估对EMT和热囊细胞入侵的影响.
调查BPTF与 Slug 基因促进物的直接结合.

主要成果:

在RM患者的小体中,BPTF表达显著下调.
发现BPTF参与了细胞粘附通路.
击败BPTF抑制了 trofhoblast EMT,并在体外减少了 trofhoblast 的入侵.
BPTF直接与 Slug 基因促进体结合,激活其转录.
无论是BPTF还是Slug蛋白水平,都在RM患者的小细胞原体中降低.

结论:

通过激活 Slug 表达,BPTF 在调节 trofhoblast EMT 中起着至关重要的作用.
在BPTF功能中的缺陷是重复性流产的病原体的一个重要因素.
BPTF是管理RM的潜在治疗点.

关键词:

从上皮细胞转变为介质细胞的过渡.这是入侵的入侵.经常发生的流产.热细胞热细胞原蛋白PHD指转录因子的转录因子

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