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Updated: Jun 30, 2025

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Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Published on: January 25, 2017
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鉴定了草鱼的介质素-23受体及其在肠道炎症中的炎症作用
Panpan Han1, Jian Tang1, Xufang Xu1
1School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, China.
International journal of biological macromolecules
|March 23, 2024
概括
介素23受体 (IL-23R) 在鱼的肠道炎症中起着关键作用. 向IL-23R为管理养殖鱼类炎症性疾病提供了一个有希望的策略.
科学领域:
- 免疫学 免疫学 免疫学
- 水产养殖是水产养殖的一种方式.
- 鱼类疾病 鱼类疾病
背景情况:
- 介素23受体 (IL-23R) 与哺乳动物的炎症性疾病有关.
- 它在远鱼炎症性疾病中的作用仍然在很大程度上未被探索.
研究的目的:
- 为了调查IL-23R在草炎症疾病中的参与.
- 探索IL-23R作为在养殖鱼类中潜在的治疗标.
主要方法:
- 克隆和特征草IL-23R的全长cDNA.
- 生成的重组蛋白 (rgcIL-23R) 和多克隆抗体 (rgcIL-23R pAb).
- 利用qPCR,细胞培养 (CIK细胞),基因淘汰和LPS刺激来评估IL-23R功能.
主要成果:
- 在Aeromonas hydrophila感染后,草鱼组织中IL-23RmRNA的调节升高.
- rgcIL-23R治疗诱导IL-17A/F1的表达;IL-23R的降低降低了IL-23R,STAT3和IL-17N的表达.
- rgcIL-23R pAb抑制了LPS诱导的IL-23/IL-17通路基因表达.
- rgcIL-23R诱导肠道炎症,而rgcIL-23R pAb则抑制了这种炎症.
结论:
- 在草中,IL-23R对调节肠道炎症至关重要.
- IL-23R是控制养殖鱼类炎症疾病的潜在治疗标.
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