乌尔索酸可缓解小鼠卵细胞中由3 - - 尼托普罗酸诱导的介质异常
Tiancang Han1, Zhaoyang Sun1, Hongbo Zhang1
1Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji 133002, China; Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China; Yanbian University, Yanji 133002, China.
Toxicology and applied pharmacology
|March 23, 2024
概括
乌尔索酸 (UA) 保护小鼠卵细胞免受3 - - 尼托普罗酸 (3-NPA) 毒性的影响. 通过改善介质成熟,线粒体功能和减少氧化应激,UA减轻了3-NPA诱导的生殖损伤.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 线粒体医学 线粒体医学
背景情况:
- 3 - 尼特罗酸 (3-NPA) 是一种来自菌的毒素,通过抑制酸脱酶,会损害卵巢功能和生殖健康.
- 乌尔索酸 (UA) 是一种植物衍生的五环三烯,具有多种生物活性.
- 卵细胞在体外成熟对于辅助生殖技术和研究至关重要.
研究的目的:
- 在实验室中评估3-NPA对小鼠卵细胞成熟的细胞毒性作用.
- 调查UA对3-NPA诱导的卵细胞损伤的保护潜力.
主要方法:
- 鼠标卵细胞在体外培养,使用和不使用3-NPA和UA.
- 评估卵细胞的介质成熟,包括极体挤出和/皮质颗粒形态.
- 评估了线粒体功能 (膜潜力,ATP),氧化应激标志物 (GSH,ROS) 和细胞死亡途径 (细胞亡,自).
主要成果:
- 3-NPA显著损害了卵细胞的成熟,导致极体挤出减少,异常的线和皮质颗粒分布.
- 3-NPA诱导了线粒体功能障碍,增加了氧化应激 (减少了GSH,ATP;增加了ROS),并触发了亡和自.
- 补充UA有效地减轻了3-NPA对卵细胞成熟和细胞健康的有害影响.
结论:
- 在体外成熟过程中,3-基酸对卵细胞发育构成重大威胁.
- 乌尔索酸显示出对3-NPA诱导的耳膜毒性有强大的保护作用.
- 氨酸有望缓解与3-NPA暴露相关的生殖障碍.
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