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Updated: Jun 30, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

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通过单基因分辨率突变分类器破译的DNA双链断裂修复的发育进展

Zhiqian Li1,2, Lang You1,2, Anita Hermann1,2

  • 1Department of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, 92093, USA.

Nature communications
|March 24, 2024
PubMed
概括

这项研究引入了一个综合分类管道 (ICP) 来分析昆虫中的CRISPR/Cas9突变. ICP揭示了不同的DNA修复路径指纹 (MMEJ,NHEJ,HDR),这些指纹在发育过程中发生变化,有助于突变跟踪.

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