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长期COVID的儿童和青少年I型干扰素信号的年龄相关的转录变化

Matteo Fracella¹, Enrica Mancino², Raffaella Nenna²

¹Department of Molecular Medicine, Laboratory of Virology, Sapienza University of Rome, Rome, Italy.

European journal of immunology

|March 24, 2024

概括

长期COVID在儿童中显示出I型干扰素 (IFN-I) 免疫反应的年龄相关变化. 与健康对照人群相比,长期COVID的儿童和青少年都表现出IFN-I信号通路的改变.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
儿科儿科儿科

背景情况:

虽然SARS-CoV-2在儿童中通常呈现温和,但持续的症状表明潜在的长期COVID (LC) 发展.
天生的免疫力,特别是I型干扰素 (IFN-I) 反应,可能在长期COVID的免疫病理学中发挥作用.
了解这些免疫变化对于诊断和管理儿科长期COVID至关重要.

研究的目的:

研究长期COVID症状的儿童和青少年I型干扰素 (IFN-I) 反应.
将儿科长期COVID患者的IFN-I通路基因表达与康复的轻度COVID-19病例和健康对照进行比较.
探索儿童群体中与长期COVID相关的免疫反应的年龄相关差异.

主要方法:

评估了IFN-I家族成员 (IFN-α/β/ε/ω),IFN-I受体组件 (IFNAR1/2) 和干扰素刺激基因 (ISG) 在外周血液单核细胞 (PBMC) 中的mRNA表达.
分析了患有长期COVID的儿童和青少年的样本 (LC; n=28),康复的轻度COVID-19 (MC; n=28) 和健康的对照 (HC; n=18),感染后3-6个月收集.
在三组之间比较基因表达水平,按年龄分层 (儿童6-11岁,青少年12-17岁) 和神经症状的存在.

主要成果:

患有LC的青少年显示IFN-β,IFN-ε和IFN-ωmRNA比HC更高,IFN-α,IFN-β和IFN-ω与MC相比增加.
与HC相比,LC患儿的IFN-β,IFN-ε和IFN-ωmRNA水平较低,IFN-α,IFN-β和IFN-ω水平也较低.
无论LC和MC组都显示ISG和IFNAR1表达的减少,但IFNAR2表达相对于HC的增加,表明IFN-I信号通路调节的改变.

结论:

儿童和青少年的长期COVID与I型干扰素信号通路的显著,年龄相关的变化有关.
这些发现突显了儿科长期COVID中独特的免疫失调模式,在幼儿和青少年之间有所不同.
需要进一步的研究,以阐明这些IFN-I途径变化的精确机制和临床影响,在儿科长期COVID.

关键词:

青少年青少年青少年孩子孩子孩子孩子这就是ISG的意义.长时间的COVID.对干扰素的中和性自身抗体在SARS-CoV-2中.这是一种I型干扰子.

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