来自牛Rhipicephalus microplus的核糖体蛋白S18的生物功能评估
Gabriel C A Costa1, Fernando A A Silva1, Ricardo J S Torquato1
1Departamento de Bioquímica, Escola Paulista de Medicina, Universidade de Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Ticks and tick-borne diseases
|March 24, 2024
概括
牛的滴答是牛的滴答.
科学领域:
- 兽医昆虫学 兽医昆虫学
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
背景情况:
- 牛 (Rhipicephalus microplus) 由于寄生和病原体传播,造成了重大的经济损失.
- 核糖体蛋白S18 (RPS18) 是40S核糖体子单元的组成部分,在蛋白质合成中具有已知的作用.
- 在生理学中RPS18的特定功能,特别是在繁殖和血液养方面,仍未得到充分研究.
研究的目的:
- 从功能上描述来自Rhipicephalus microplus的核糖体蛋白S18 (RPS18) 的特征.
- 调查RmRPS18在的血液养,卵子生产和卵子活力的作用.
- 评估RmRPS18作为新控制策略的目标的潜力.
主要方法:
- 在血液养期间,在雌性中分析RmRPS18的基因表达.
- 在牛血清中检测抗RmRPS18抗体.
- 在成年雌性中,RNA干扰 (RNAi) 抑制RmRPS18表达.
- 评估复合RmRPS18.18的卵产量,活力和抗菌活性.
主要成果:
- 在血液养期间,唾液腺体的RmRPS18基因表达显著上调.
- 再组合RmRPS18被感染牛血清的抗体识别出来.
- 通过RNAi介导的RmRPS18的淘汰导致卵产量减少29%,卵完全受损.
- 再组合RmRPS18对大肠杆菌和黄金葡萄球菌表现出抗菌活性.
结论:
- RmRPS18在Rhipicephalus microplus的繁殖中起着至关重要的作用,影响卵子的生产和活力.
- RmRPS18在牛中具有免疫性,这表明宿主对这种蛋白质的免疫反应.
- 抗菌性质和生殖中的重要作用表明RmRPS18是开发新的牛控制方法的有希望的目标.
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