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Updated: Jun 30, 2025

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新的候选基因可能参与寨卡病毒产

Miriãn Ferrão Maciel-Fiuza¹, Bruna Duarte Rengel², Gabriela Elis Wachholz²

¹Graduate Program in Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Instituto Nacional de Genética Médica Populacional, Porto Alegre, Brazil; Genomics Medicine Laboratory, Center of Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.

Computers in biology and medicine

|March 24, 2024

概括

研究人员确定了包括PRKDC,PCNA,ATM,SMC3,FGF8和SHH在内的关键基因,在寨卡病毒诱导的大脑形中表达变化. 系统生物学方法有助于确定这些关键基因用于先天性寨卡综合征研究.

科学领域:

神经科学是一个神经科学.
病毒学病毒学
遗传学是一个遗传学.

背景情况:

寨卡病毒 (ZIKV) 致使人生,导致先天性寨卡综合征 (CZS),但其分子病原性仍然不清楚.
了解ZIKV诱导的大脑发育问题的遗传基础对于开发干预措施至关重要.

研究的目的:

为了确定与大脑发育相关的基因,这些基因可能会受到ZIKV感染的影响.
阐明CZS病原体背后的分子机制.

主要方法:

基因数据库和蛋白质与蛋白质相互作用网络的生物信息学分析.
在ZIKV诱导的先天性寨卡综合征动物模型中的基因表达分析.
在暴露于ZIKV.病毒的人类细胞中的差异基因表达 (DGE) 分析.

主要成果:

总共有2610个与大脑发育和CZS相关的基因被确定,其中1013个形成了一个连接的网络.
使用网络中心性统计数据选择了9个候选基因.
在CZS模型中观察到PRKDC,PCNA,ATM,SMC3,FGF8和SHH的明显表达模式,ATM,PRKDC和PCNA在人体细胞中表达变化.

结论:

系统生物学有效地识别了ZIKV诱导的大脑形的候选基因.
确定PRKDC,PCNA,ATM,SMC3,FGF8和SHH是ZIKV相关大脑异常中表达变化的关键基因,需要进一步调查.

关键词:

生物信息学是一种生物信息学.有先天性异常.胚胎学胚胎学基因表达特征分析微头症是一个微头症.由ZIKV诱导的梯形发生.

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