通过共价和非共价相互作用通过EGCG对大豆蛋白分离物-可溶性大豆多糖化合物的修饰机制:结构,界面和功能性质
1College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Food chemistry
|March 24, 2024
概括
这项研究开发了一种使用大豆蛋白分离物,多糖和多 (EGCG) 的新型天然乳化剂. 由此产生的三元复合体表现出增强的抗氧化和乳化特性,使其非常适合食品和制药应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 天然乳化剂是作为合成乳化剂的替代品被寻求的.
- 大豆蛋白分离物 (SPI) 具有乳化特性,但可以改进.
- 聚,像EGCG,提供抗氧化功效,并可以修改蛋白质结构.
研究的目的:
- 通过修改SPI与多糖和EGCG,创建具有抗氧化能力的天然乳化剂.
- 研究EGCG度和相互作用类型 (共价/非共价) 对SPI-SSPS复合物的特性的影响.
- 评估新型三元复合体的物理化学,结构,界面和功能特征.
主要方法:
- 使用不同度的EGCG制备SPI-SSPS-EGCG三元复合物.
- 物理化学性质的分析:绝对潜力,度,表面水性.
- 使用对秩序/混乱敏感的技术评估结构变化.
- 界面属性的评估:乳化活性指数 (EAI) 和乳化稳定性指数 (ESI).
- 测量抗氧化能力的测量.
主要成果:
- 低度的EGCG增加了潜力并降低了度,而较高度则增强了EGCG的结合性并降低了疏水性.
- EGCG改变了SPI-SSPS的结构;共价相互作用导致有序结构,非共价导致无序结构.
- 共价的SPI-SSPS-EGCG复合体显示,随着EGCG的增加,EAI和ESI显著增加.
- 非共价复合体显示了显著的EAI变化,但不是ESI.
- 三元复合体显著提高了抗氧化能力.
结论:
- SPI-SSPS-EGCG三元复合物是一种有前途的天然乳化剂,具有卓越的抗氧化和乳化功能.
- 它的特性可以通过控制EGCG度和相互作用类型来调整.
- 这种新型复合物有可能用于先进的乳液输送系统.
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