在红光调节的植物染色体中,以动力驱动的性激活迪加尼酸环酶活性
Quang Hieu Tran1, Oliver Maximilian Eder1, Andreas Winkler2
1Institute of Biochemistry, Graz University of Technology, Graz, Austria.
The Journal of biological chemistry
|March 24, 2024
概括
动力驱动的全是信号集成的关键在细菌菌体中. 变体中变化的蛋白质动态与酶活性相关,揭示了信号处理和传感器效应器设计的洞察力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 传感器效应蛋白质将刺激整合到细胞反应中.
- 细菌菌体是模块化的红光响应蛋白,调节各种效应因子,包括GGDEF diguanylate cyclases.
- 缺乏对长距离信号处理在植物染色体 diguanylate 循环酶的概括理解.
研究的目的:
- 阐明在植物染色体 diguanylate 循环中信号整合和通信的分子机制.
- 研究蛋白质动态在全调节中的作用.
- 了解不同形态状态和二元配置的功能意义.
主要方法:
- 蛋白质变体的生成在Pfr状态中稳定.
- 使用-交换与质谱学 (HDX-MS) 结合的结构动力学的分析.
- 酶活性测定和快速逆转变种的表征.
主要成果:
- 单氨基酸替代剂改变了蛋白质动态,这与酶活性相关.
- 变化的动力学解释了非光色变异的活性增加.
- 混合Pfr/中间状态二极体在功能上很重要,它们支持植物染色体中的单质子激活.
结论:
- 动力驱动的全ostery对于细菌菌体中信号集成至关重要.
- 蛋白质的结构动态直接影响酶活性和信号传导.
- 这些发现为设计用于光遗传学的新型细菌基传感器效应器系统提供了基础.
更多相关视频
09:32Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
8.7K
09:40Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
Published on: September 20, 2011
18.0K
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Photoreceptors and Plant Responses to Light
20.3K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.3K
Activation and Inactivation of G Proteins
7.1K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
7.1K
Biological Clocks and Seasonal Responses
34.6K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.6K
GPCRs Regulate Adenylyl Cylase Activity
5.5K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.5K
Channel Rhodopsins
2.6K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.6K
