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来自人类和老鼠的分子生成的老鼠肝炎E病毒菌株在人类肝瘤细胞系中表现出高效的复制

Jessica Panajotov¹, Alexander Falkenhagen¹, Ashish K Gadicherla²

¹German Federal Institute for Risk Assessment, 10589 Berlin, Germany.

|March 24, 2024

概括

鼠类肝炎E病毒 (ratHEV) 可以感染人类肝细胞,表明有动物感染的潜力. 需要进一步的研究来确定允许的老鼠细胞,以了解老鼠HEV的致病性.

科学领域:

病毒学病毒学
肝病学肝病学是一种肝病学.
动物传播疾病动物传播疾病

背景情况:

肝炎E病毒 (HEV) 导致人类肝炎.
曾经认为,老鼠HEV (Rocahepevirus ratti) 感染仅限于老鼠.
最近的人类病例表明鼠HEV的动物传播.

研究的目的:

为了研究老鼠HEV.rat的动物感染潜力.
构建和利用大鼠HEV的基因组克隆用于体外研究.
评估老鼠HEV在人类和老鼠肝瘤细胞系中的感染性和复制性.

主要方法:

构建了大鼠HEV菌株 (pt2,R63) 和人类HEV基因型3的基因组克隆.
在体外转录的RNA被转移到人类肝瘤细胞系 (HuH-7-Lunet BLR,HuH-7,PLC/PRF/5) 和大鼠肝瘤细胞系.
通过量化基因组拷贝数和检测病毒抗原来监测病毒复制.

主要成果:

拉特HEV菌株在HuH-7-Lunet BLR细胞中有效复制,建立了持久性感染.
人类肝瘤细胞系显示出对老鼠HEV感染的可变敏感性.
来自老鼠的肝瘤细胞系不支持有效的老鼠HEV复制.

结论:

无论是来自老鼠还是人类的RatHEV菌株都能感染人类肝瘤细胞.
复制效率取决于特定的细胞系和病毒菌株.
需要进行进一步的研究,以确定用于ratHEV研究的允许性大鼠细胞系.

关键词:

一个基因组克隆.肝炎E病毒的感染.肝瘤细胞系的细胞系人类人类人类人类人类鼠标鼠标鼠标鼠标动物传染病的潜在潜力

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