在NOBOX中的多态性和子大小特征在苏猪中的关联
Jiajin Huang1,2,3, Yong Ruan1,2,3, Meimei Xiao1,2,3
1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China.
Frontiers in veterinary science
|March 25, 2024
概括
新生卵巢家庭盒基因 (NOBOX) 在苏猪的多态性与子大小有关. 特定的NOBOX基因突变影响蛋白质功能和生殖特征,为养猪提供了洞察力.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 遗传学 是一个
- 动物科学动物科学
背景情况:
- 新生儿的卵巢家庭盒基因 (NOBOX) 对于卵巢和卵细胞的发育至关重要,在哺乳动物的繁殖中发挥着至关重要的作用.
- 了解影响子大小的遗传因素对于提高猪养殖效率至关重要.
研究的目的:
- 为了研究NOBOX在各种苏猪组织中的mRNA表达.
- 在NOBOX基因中识别单核酸多态 (SNP) 并分析它们与 litter 尺寸特征的关联.
- 用生物信息学评估NOBOX误解突变对蛋白质结构和功能的影响.
主要方法:
- 定量实时PCR (qRT-PCR) 用于mRNA表达分析.
- 用DNA测序来检测NOBOX基因多态性 (SNP).
- 统计分析以将NOBOX SNPs与 litter 尺寸相关联.
- 生物信息工具用于预测误解突变的功能影响.
主要成果:
- NOBOX在苏猪的卵巢中表现出偏好的mRNA表达.
- 在NOBOX基因序列中发现了6种突变,其中g.1858 G>A是一个错误的突变.
- g.1858 G>A,g.5659 A>G和g.6025 T>A的SNP与 litter 尺寸有显著的相关性 (p <0.05).
- 误解突变g.1858 G>A (p.V82M) 预计是非保守的,减少蛋白质的稳定性和改变蛋白质的功能.
结论:
- NOBOX基因多态性与子大小显著相关.
- 鉴定到的NOBOX突变,特别是g.1858 G>A,可能会通过改变蛋白质功能来影响生殖能力.
- 这些发现提供了有价值的遗传标记,用于提高养猪计划中的子大小.
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