在废水处理厂的初级废水中,SARS-CoV-2的固体-液体分布
Femi F Oloye1, Yuwei Xie1,2,3, Mohsen Asadi4
1Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.
MethodsX
|March 25, 2024
概括
了解严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 在废水固体中的分布至关重要. 这项研究显示,SARS-CoV-2更喜欢固体,而便标记者更喜欢液体,为基于废水的流行病学提供了信息.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 基于废水的流行病学 (WBE) 对于监测公共卫生至关重要,包括跟踪SARS-CoV-2.
- 了解废水阶段的病毒分布对于准确的WBE数据至关重要.
- 关于SARS-CoV-2和废水固体与液体中的便生物标志物分区的数据有限.
研究的目的:
- 确定SARS-CoV-2和便病毒生物标志物 (PMMoV,FRNAPH-II) 在固体和液体废水阶段之间的分布.
- 为了比较不同废水分成方法对病毒恢复的有效性.
- 评估RNA提取套件对病毒RNA产量和PCR抑制的影响.
主要方法:
- 使用RT-qPCR进行病毒RNA提取和量化.
- 三种废水分化技术的比较:膜过,中速离心加膜过和高速离心.
- 评估两个RNA提取工具包:RNeasy PowerWater Kit和QIAamp病毒RNA迷你工具包.
主要成果:
- SARS-CoV-2 主要通过特定的分成方法分成固体部分,而便生物标志物显示相反的趋势.
- 废水分成方法对测试的病毒和菌体标记物的固体-液体分布的影响最小.
- 与QIAamp病毒RNA迷你套件相比,RNeasy PowerWater套件产生了更高的病毒RNA负载和降低了PCR抑制.
结论:
- 在废水固体中SARS-CoV-2的分布是显著的,并且取决于分化方法.
- 对PCR抑制的有效管理对于可靠的基于废水的流行病学至关重要.
- 选择RNA提取套件显著影响病毒RNA恢复和WBE精度.
关键词:
在 COVID-19 疫情中,环境RNA 是一种环境RNA.在废水中进行SARS-CoV-2RNA分离的方法开发.PCR 抑制剂 PCR 抑制剂监督监督监督监督监督监督监督监督监督监督监督监督基于废水的流行病学.更多相关视频
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