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使用微卫星和SNP标记物的两种罕见树物种的现场保护

Austin C Koontz¹, Emily K Schumacher¹, Emma S Spence^1,2

¹Morton Arboretum Center for Tree Science Lisle Illinois USA.

Evolutionary applications

|March 25, 2024

概括

植物园中的植物收藏对 ex situ 保护至关重要. 新的SNP数据证实,基于微观卫星的样本大小一般适用于维持这些重要植物集合中的遗传多样性.

科学领域:

保护生物学保护生物学
遗传学是一个遗传学.
植物学植物学

背景情况:

植物园中的ex situ保护收藏对于保持植物遗传多样性至关重要.
目前对遗传多样性的样本大小建议通常基于微观卫星标记物.
这些建议在使用较新的下一代测序 (NGS) 方法时的有效性尚不清楚.

研究的目的:

为了比较现场保护状况和采样建议,使用微卫星与单核酸多态 (SNP) 相比.
评估数据过和SNP调用方法对现场表示的影响.
评估SNP数据对解决人口结构和分配ex situ个体的有用性.

主要方法:

分析了两种木 (Quercus acerifolia 和 Q. boyntonii) 受到威胁的园林和野生样本.
从微观卫星和SNP数据获得的遗传多样性指标的比较.
评估不同的数据过和SNP调用策略.
使用两种标记类型评估种群差异化和结构.

主要成果:

与微卫星相比,SNP表明Quercus acerifolia的ex situ代表性较低,样本大小略小.
对于Quercus boyntonii的标志物结果是相似的.
缺少的数据过器通常会增加现场表现.
SNP数据提供了更高分辨率的人口结构和个体分配.

结论:

微卫星和SNP标记物都可用于评估ex situ集合中的遗传多样性.
SNP数据为人口结构和个人分配提供了增强的分辨率.
为在现场保护中使用SNP数据提供了指导,包括数据过和参考基因组使用.

关键词:

生物信息学/植物信息学囚禁种群中的囚禁种群.保护生物学保护生物学保护遗传学保护遗传学人口遗传学经验性遗传学

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