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新的长非编码RNA生物标记物和ceRNA网络在结直肠癌中的miR-616-3p:基于生物信息学的研究

Mohammad Abdolvand^1,2,3, Zahra Mohammadi Chermahini³, Sahar Bahaloo⁴

¹Cellular, Molecular and Genetics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences

|March 25, 2024

概括

这项研究确定了新型长非编码RNAs (lncRNAs) 和信使RNAs (mRNAs) 作为结直肠癌 (CRC) 的潜在生物标志物. 这些发现揭示了新的调控网络,涉及用于CRC诊断和治疗的microRNA (miRNA).

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
癌症研究癌症研究

背景情况:

长非编码RNAs (lncRNAs) 作为竞争的内源RNAs (ceRNAs) 起作用,隔离microRNAs (miRNAs) 并影响基因表达.
通过lncRNA介导的ceRNA网络的失调与各种癌症的发展有关,包括结直肠癌 (CRC).

研究的目的:

确定结直肠癌 (CRC) 中的一种新型调节轴,涉及微RNA-616-3p (miR-616-3p).
发现基于lncRNA和mRNA表达的CRC潜在的诊断和预后生物标志物.
阐明CRC中尚未探索的ceRNA网络.

主要方法:

利用基因表达综合数据库识别CRC中的差异表达lncRNAs (DELs) 和mRNAs.
使用生物信息学工具 (RegRNA,TargetScan,ciBioPortal,Ensemble) 来预测RNA相互作用并构建ceRNA网络.
使用定量实时聚合酶链反应 (qRT-PCR) 验证了关键RNA分子的表达水平.

主要成果:

确定Linc01282,lnc-MYADM-1:1,和指蛋白347 (ZNF347) 在CRC中显著过度表达.
在CRC中发现盐诱导性激酶1 (SIK1) 和miR-616-3p的下调.
证实了CRC组织中特定的lncRNAs,mRNAs和miRNAs的失调.

结论:

发现了新的,未报告的 lncRNAs,有可能作为CRC的预后生物标志物.
确定了潜在的mRNA作为潜在的治疗点和CRC的预测生物标志物.
发现了以前未经探索的ceRNA网络,需要进一步研究CRC病变的发生.

关键词:

结肠直肠癌是一种癌症.在ZNF347中,我们可以看到ZNF347.联系人:linc0128282 在线咨询在MYADM-1:1中.微型RNAs-616-3pp的使用情况盐诱导性激酶 1 盐诱导性激酶 1

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