多个离子隔离和积累事件用于选择性化学噪声降低和MALDI成像质谱仪中的动态范围增强
Troy R Scoggins1, Jonathan T Specker1, Boone M Prentice1
1Department of Chemistry, University of Florida, Gainesville, FL, USA. booneprentice@chem.ufl.edu.
The Analyst
|March 25, 2024
概括
在MALDI成像质谱仪中,化学噪声和空间电荷效应阻碍了检测. 选择性离子的多次连续积累 (Multi CASI) 有效地降低了噪音,并改善了大鼠大脑组织中代谢物和脂质的检测.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 质谱测量质量谱测量
背景情况:
- 矩阵辅助激光脱/电离 (MALDI) 成像质谱 (IMS) 对于分析组织中的生物分子分布至关重要.
- 高度的矩阵离子和内源化合物会产生化学噪声,限制低丰度分子的检测.
- 马尔迪-IMS中的离子捕获仪器可能会受到空间电荷的影响,从而降低动态范围并阻碍检测重要的低丰度化合物.
研究的目的:
- 为 MALDI-IMS. 引入和描述一种新的气相分离技术,即选择的离子的多重连续积累 (Multi CASI),用于 MALDI-IMS.
- 为了证明Multi CASI在减少化学噪声和减轻空间充电效应方面的有效性.
- 提高生物组织分析中低丰度代谢物和脂质的检测.
主要方法:
- 使用四极质量过器对特定离子进行序列隔离.
- 在质量分析之前,实施了选择的离子的多次连续积累 (Multi CASI).
- 应用了Multi CASI技术到MALDI对大鼠大脑组织的成像质谱学实验.
主要成果:
- 成功地减少了源自MALDI矩阵和丰富的内源化合物的化学噪声.
- 显著减少空间充电效应,从而改善了仪器的动态范围.
- 实现了对低丰富度代谢物和脂质的增强检测和改善的信号噪声比.
结论:
- 选择性离子的多次连续积累 (Multi CASI) 是MALDI-IMS中降低噪声的有效策略.
- 这种方法提高了灵敏度和动态范围,可以更好地检测低丰度分析物.
- 多种CASI代表了MALDI-IMS在生物组织分析中的应用,特别是对代谢物和脂质分析的宝贵进步.
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