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结晶和现场室温数据采集使用哈维尔的结晶设施和Beamline VMXi,钻石光源

James Sandy1, Halina Mikolajek2, Amy J Thompson1

  • 1Diamond Light Source Ltd, Harwell Science and Innovation Campus.

Journal of visualized experiments : JoVE
|March 25, 2024
PubMed
概括

机器人蛋白质结晶和室温X射线数据收集能够快速,高质量的结构确定. 这个自动化管道为全球研究人员提供了结构生物学洞察力,了解蛋白质动力学和连接体结合.

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Determination of Crystal Structures01:29

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In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...