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一种超敏感的基因编码电压指示器揭示了不可激发细胞的电活动

Philipp Rühl¹, Anagha G Nair¹, Namrata Gawande¹

¹Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena and Jena University Hospital, D-07745, Jena, Germany.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|March 25, 2024

概括

科学家们开发了rEstus,这是一种高度敏感的电压传感器,用于不可激发的细胞. 这种工具揭示了先前未被研究的细胞类型的自发电活动和细胞-细胞通信.

科学领域:

细胞生物学细胞生物学
神经科学是一个神经科学.
生物物理学的生物物理.

背景情况:

无法激发的细胞缺乏动作潜力,需要高度敏感的电压传感器.
现有的电压传感器很难在不可激发的电池中检测微妙的电压变化.

研究的目的:

设计一个超敏感,明亮,可校准的基因编码电压传感器 (rEstus).
为了研究非刺激细胞中自发的膜电压变化.
用光学电压记录来评估电气电池-电池合.

主要方法:

一个新的遗传编码电压传感器的结构引导工程,rEstus.
使用rEstus进行高灵敏度电压成像,用于不可激发的细胞系 (A375,HEK293T,MCF7).
对光学记录的电压信号进行相关性分析,以确定电池合.

主要成果:

与其前身相比,rEstus在非激发细胞的静止电压范围内表现出优越的亮度和灵敏度.
在A375,HEK293T和MCF7细胞中检测到自发的内源膜电压波动,时间尺度为秒到毫秒.
在A375和HEK293T细胞中观察到视觉和电气合之间的直接相关性,在MCF7细胞中合较弱.

结论:

rEstus提供了一种增强的工具,用于在活的不可激发细胞中进行非侵入性电压成像.
自发的膜电压变化是各种不可激发的细胞类型中常见的现象.
光学电压记录提供了电池-电池通信的直接读取.

关键词:

细胞膜潜在的细胞膜.电力生理学电力生理学光成像成像技术的使用.遗传编码的电压指示器不能激发的细胞.

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