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Updated: Jun 29, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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用冷电子显微镜净化单链RNA细菌和宿主受体进行结构确定

Jirapat Thongchol1, Junjie Zhang2

  • 1Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.

Methods in molecular biology (Clifton, N.J.)
|March 25, 2024
PubMed
概括

单链RNA菌体 (ssRNA菌体) 是各种领域的重要工具. 这项研究详细介绍了一种用于净化MS2和Qβ菌体及其F-pilus受体的方法,用于冷电子显微镜结构分析.

关键词:
化电磁波是一种冷电磁波.在F-pilus中使用F-pilus.在MS2中,MS2是MS2.Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ Qβ QβssRNA 菌体是什么

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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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