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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 29, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

使用ONT-Cappable-Seq获得详细的菌体转录组.

Leena Putzeys¹, Danish Intizar¹, Rob Lavigne²

¹Laboratory of Gene Technology, Department of Biosystems, KU Leuven, Leuven, Belgium.

Methods in molecular biology (Clifton, N.J.)

|March 25, 2024

概括

ONT-cappable-seq使用纳米孔测序实现了详细的菌体转录组学. 这种方法提供了对菌体转录的全面概述,改善了分子菌体生物学理解和工程潜力.

科学领域:

微生物学微生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

关键词:

细菌菌体是一种细菌.纳米孔测序的测序我们的操作人员.活动主办人的身份识别.终结者终结者终结者终结者文字转录学 (Transcriptomics) 是一个学科.

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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

相关实验视频

Last Updated: Jun 29, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

菌体转录地图未被充分探索,阻碍了分子菌体生物学洞察力.
有限的理解限制了菌体利用和工程应用.

结论:

ONT-cappable-seq为详细的菌体转录分析提供了一个强大的工具.
增强对分子菌体生物学和工程能力的理解.
铺平了细菌转录的全面概述的道路.