酸化,乱和相位分离控制着真菌昼夜时钟中频率的行为
Daniyal Tariq1, Nicole Maurici2, Bradley M Bartholomai3
1Department of Chemistry & Chemical Biology, Cornell University, Ithaca, United States.
eLife
|March 25, 2024
概括
在Neurospora crassa中,频率 (FRQ) 蛋白质形成了一个调节基因表达的复合体. 这种内在无序的蛋白质经历了液体-液体相分离,这种过程可能存在于真核生物钟中.
科学领域:
- 分子生物学分子生物学
- 生物物理学的生物物理.
- 时间生物学 时间生物学
背景情况:
- 循环时钟通过转录-翻译反循环调节基因表达.
- 在Neurospora crassa中,频率 (FRQ) 蛋白,FRQ相互作用的RNA基酶 (FRH) 和酶-酶I (CK1) 构成FFC复合体,抑制由白领复合体 (WCC) 激活的基因.
研究的目的:
- 为了研究内在无序的FRQ蛋白和FFC复合物的结构和动态.
- 了解液-液相分离 (LLPS) 在昼夜时钟的功能中的作用.
主要方法:
- 用自旋标记和脉冲二极电子自旋共振光谱来研究FRQ和FFC复合体.
- 进行了Neurospora hyphae的实时成像,以观察FRQ局部化.
主要成果:
- FRQ包含一个紧的核心,组织FRH和CK1用于WCC压制.
- FRQ酸化改变了它的结构和动态,促进了LLPS.
- FRQ通过LLPS将FRH和CK1隔离起来,影响CK1的活动,并在核外围附近形成焦点.
- 在较高的温度下,LLPS有负反,以减少FRQ化.
结论:
- 通过LLPS形成凝结物可能是真核生物昼夜钟的保存机制,尽管其低序列同质性.
- FRQ的内在无序性质对于其在组织FFC复合体和调节昼夜节律方面的功能至关重要.
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