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对不同表达的lncRNA和mRNA与缓慢过渡便秘相关的分析

Yuanxin Miao¹, Xiongwei Xie², Yonglian Zhang²

¹Research Institute of Agricultural Biotechnology, Jingchu University of Technology, Jingmen 448000, China.

|March 25, 2024

概括

研究人员确定了关键的信使RNAs (mRNAs) 和长非编码RNAs (lncRNAs) 与缓慢过渡便秘 (STC) 相关,这是一个具有挑战性的胃肠道疾病. 这项研究揭示了STC.

科学领域:

胃肠道学和分子生物学
基因组学和转录基因组学

背景情况:

慢过渡性便秘 (STC) 是一种难以治疗的胃肠道疾病,影响了大量慢性便秘患者.
目前尚不清楚STC的基本分子机制,这阻碍了开发有效治疗方法.

研究的目的:

识别与STC相关的关键差异表达 (DE) 传递 RNA (mRNA) 和长非编码 RNA (lncRNA).
探索涉及STC病变发生的分子途径和潜在生物标志物.

主要方法:

从STC患者和对照组的西格状肠组织的高通量RNA测序.
定量实时PCR用于验证DE lncRNAs和mRNAs.
权重基因共同表达网络分析 (WGCNA) 和皮尔森相关性分析以确定mRNA-lncRNA相互作用.
基因和基因组的京都百科全书 (KEGG) 途径分析和蛋白质-蛋白质相互作用 (PPI) 网络构建.

主要成果:

总共有1420个DE lncRNA和1634个DE mRNA被确定.
凯格分析表明,与免疫相关的途径,如炎症性肠病和NF-kappa B信号传递有关.
通过WGCNA和相关性分析,确定了16,577个显著相关的DE mRNA-lncRNA对,其中LINC00641,LINC02268和LINC03013被突出显示为枢纽 lncRNA.
对PPI网络的分析表明,干白素2 (IL2),CD80分子 (CD80) 和干白素-17A (IL-17A) 在STC的发展过程中都涉及.

结论:

本研究提供了对STC中的lncRNA和mRNA表达特征的全面分析.
鉴定的基因和途径为STC的分子机制提供了洞察力.
这些发现可能会导致开发新的STC诊断和治疗生物标志物.

关键词:

长非编码RNA (LncRNA) 是一种长非编码RNA.这是一个PPI PPI.RNA测序 (RNA-seq) 是指RNA的测序.缓慢过渡性便秘缓慢过渡性便秘在WGCNA中,WGCNA是WGCNA.

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