间歇性禁食改变了日间转录因子的转录因子图书馆
Min Fu1, Siyu Lu2,3, Lijun Gong2,3
1Department of Neurology, The Fourth Hospital of Changsha, Affiliated Changsha Hospital of Hunan Normal University, Changsha, 410006, Hunan, China.
Molecular and cellular biochemistry
|March 26, 2024
概括
间歇性禁食 (IF) 改变了组织中转录因子 (TF) 的日常节奏. IF改变了TF表达,通过抑制mTOR信号传递和影响衰老途径,可能会带来健康益处.
科学领域:
- 代谢研究的研究.
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 间歇性禁食 (IF) 是一种用于管理与年龄有关的疾病的公认策略,但其潜在的分子机制需要进一步阐明.
- 转录因子 (TFs) 在调解细胞对环境线索的反应中发挥着至关重要的作用,包括饮食变化.
- 了解TFs的动态调节对于破译IF的生理影响至关重要.
研究的目的:
- 在各种养方案下,在多种代谢组织中创建一本日转录因子 (TF) 表达的综合图谱.
- 研究间歇性禁食如何影响TFs的时间和组织特异性表达模式.
- 探索TF波动,食模式和与年龄相关的健康益处之间的关系.
主要方法:
- 在24小时内,每4小时收集四个小鼠代谢组织的600个样本,对日间TF表达的概况.
- 在五种不同的食方案中对TF表达特征进行比较,包括随意食和间歇性禁食协议.
- 生物信息分析以确定组织特异性,时间振荡和对饮食反应的TFs.
主要成果:
- 在任意养小鼠中鉴定出1218个具有组织特异性和时间表达的TF,其中974个表现出显著的日间振荡.
- 观察到间歇性禁食在90%以上的TF中引起了振荡,并改变了它们的组织特异性表达模式.
- 发现IF在单次禁食后通常在骨肌肉和脂肪组织中促进TF表达,但在持续的IF中总体抑制了TF表达,降低了衰老途径的调节,并提高了mTOR抑制途径的调节.
结论:
- 间歇性禁食显著重塑了日间转录组转录因子的地图.
- 观察到的TF表达的转变,特别是对哺乳动物目标拉巴胺素 (mTOR) 信号的抑制,可能是IF介导的健康改善的关键机制.
- 生成的TF图谱是了解TFs,昼间节律和间歇性禁食的健康益处之间的相互作用的宝贵资源.
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