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鉴定和功能分析的叶子基因在长花诱导诱导

Dengwei Jue^1,2, Zhexin Li¹, Wenlin Zhang¹

¹Chongqing Key Laboratory of Economic Plant Biotechnology, Collaborative Innovation Center of Special Plant Industry in Chongqing, Chongqing Engineering Research Center for Special Plant Seedling, Institute of Special Plants, Chongqing University of Arts and Sciences, 402160, Yongchuan, China.

|March 26, 2024

概括

研究人员确定了Dimocarpus longan LEAFY (DlLFY) 基因,这是龙开花的关键调节者. 在Arabidopsis中过度表达DLFY促进了早期开花,并表明与TFL1的相互作用,这对长期产量至关重要.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学

背景情况:

开花时间对于稳定的长期产量至关重要.
了解长期开花法规对于行业发展至关重要.
控制龙开花的关键基因在很大程度上是未知的.

研究的目的:

识别了调节龙开花的关键基因.
分析龙开花的调节机制.
为科学研究和长期行业进步提供洞察力.

主要方法:

基因克隆和DLLFY的序列分析.
在各种长组织和不同开花阶段进行表达分析.
亚细胞局部化和交换活化试验.
在Arabidopsis中进行过度表达的研究.
的DNA亲和力净化测序 (DAP-Seq).

主要成果:

DlLFY基因编码了一个具有典型LFY/FLO域的转录因子.
DlLFY在长长的花中高度表达,并在早期的花诱导过程中得到上调.
在Arabidopsis中过度表达DlLFY加速了开花和减少了植物大小.
DAP-Seq确定了假定目标基因,包括在转基因系中强烈抑制AtTFL1.

结论:

DlLFY在控制长花期方面发挥着至关重要的作用.
DlLFY可能通过与TFL1.1的相互作用来调节长的开花.

关键词:

叶子基因叶子基因这就是DAP-Seqq.功能性识别功能性识别长 (Dimocarpus longan) 是一个长.

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