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血清中COMP和ADAMTS7的诊断值用于椎间盘退化
Jing-Yu Ding1,2, Xu Yan1,2, Ren-Jie Zhang1,2
1Department of Orthopedics and Spine Surgery, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, China.
European journal of medical research
|March 26, 2024
概括
软骨寡合基质基质蛋白 (COMP) 和ADAMTS7作为椎间盘退行性疾病 (IVDD) 的生物标志物非常有前途. 血清中高水平的COMP和ADAMTS7与IVDD的进展相关,可以帮助诊断这种情况.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 整形外科 整形外科 整形外科
背景情况:
- 椎间盘退化 (IVDD) 是致病率和残疾的一个重要原因.
- 确定IVDD可靠的生物标志物对于早期诊断和管理至关重要.
研究的目的:
- 作为IVDD的潜在生物标志物,研究软骨寡合基质基质蛋白 (COMP) 和ADAMTS7.
- 在IVDD的背景下探索COMP和ADAMTS7之间的功能关系.
主要方法:
- 从IVDD子模型和由Pfirrmann等级分层的人类患者收集的IVD组织和外周血液.
- 使用定量方法分析了COMP和ADAMTS7表达式.
- 通过线性回归和接收器操作曲线 (ROC) 分析评估生物标志物特征.
- 在人体细胞分离物上进行了体外测试.
主要成果:
- 在子模型中,COMP和ADAMTS7表达在组织和血清中增加,IVDD进展.
- 血清中COMP (sCOMP) 和血清ADAMTS7 (sADAMTS7) 水平显示,IVD损伤后的时间依赖性增加.
- 在人类中,sCOMP,sADAMTS7和Pfirrmann等级之间发现了显著的正相关性.
- 结合的sCOMP和sADAMTS7测定,与单个标志物相比,IVDD的诊断准确性得到了提高.
- 在体外,COMP抑制了细胞外基质的降解,并对抗ADAMTS7的表达,但在IVDD模仿条件下,这种效果会减弱.
结论:
- 循环中高的COMP和ADAMTS7水平与IVDD进展相关,可能具有调节作用.
- 血清COMP和ADAMTS7测定可以区分健康个体和IVDD患者.
- 需要进一步对sCOMP和sADAMTS7作为IVDD诊断工具进行临床评估.
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