相关实验视频

Updated: Jun 29, 2025

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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细胞驱动的Cas9核酶的定向进化

Giulia Vittoria Ruta1, Matteo Ciciani2,3, Eyemen Kheir2

  • 1Laboratory of Molecular Virology, Department CIBIO, University of Trento, Trento, Italy. giuliavittoria.ruta@unitn.it.

Genome biology
|March 26, 2024
PubMed
概括

科学家们开发了一个真核生物平台来改善病例活动 (EPICA),以增强基因组编辑工具. 这个平台成功地改善了Campylobacter jejuni Cas9 (CjCas9) 核酶,在哺乳动物细胞中产生了具有显著更高活性和特异性的UltraCjCas9.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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