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转录活跃的染色质环含有"活跃"和"不活跃"的基因组修饰,在核素组群水平上表现出独家性
Stefan A Koestler1, Madeleine L Ball1, Leila Muresan2
1Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Site, Cambridge, CB2 3DY, UK.
像H3K4me3,H3K27me3和H3K36me3这样的质子修饰在Drosophila Y循环中聚集. 这些集群表现出独家性,表明在个别集群层面对基因表达的调节.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 染色质状态通过基因素修饰和组织影响DNA调节功能.
- 这些因素调节基因表达的确切关系尚不清楚.
- 德洛索菲拉Y循环是大型的,转录性活跃的染色体纤维,可分解为单个转录单元.
研究的目的:
- 为了研究沿着Drosophila Y循环的特定质子修饰的空间组织.
- 为了确定基质子修饰如何与染色质组织和转录活性有关.
- 探索在单个染色质集群水平上的调节机制.
主要方法:
- 使用超高分辨率显微镜对Drosophila melanogaster主要精子细胞Y循环进行成像.
- 分析了基因素修饰 (H3K4me3,H3K27me3,H3K36me3) 的局部化和聚类模式.
- 与RNA聚合酶II的关联被评估为特定的基因素修饰.
主要成果:
- 基质子修饰H3K4me3,H3K27me3和H3K36me3沿Y循环形成集群域.
- 不同的修饰与不同的染色质集群大小和形态相关.
- 个别的染色质集群主要表现出一种单一类型的基因素修饰,表明独家性.
- H3K36me3与积极延长的RNA聚合酶II有关,通常在循环区域.
结论:
- 基因组修饰集群和染色质集群内的独家性提供了基因调节的机制.
- 研究结果提供了有关染色体状态,组织和转录之间的相互作用的见解.
- 对理解转录性暂停,拼接和聚合酶动态的潜在影响.
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