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在染色体ZZ的斑马鱼中,直接的男性发育

Catherine A Wilson¹, Peter Batzel¹, John H Postlethwait¹

¹Institute of Neuroscience, University of Oregon, Eugene, OR, United States.

Frontiers in cell and developmental biology

|March 26, 2024

概括

斑马鱼 (Danio rerio) 的性别确定因菌株而异. 纳迪亚菌株ZZ雄性直接发育丸,而ZW雌性发育卵巢,揭示了生殖腺发育的关键遗传因素.

科学领域:

发展生物学发展生物学
遗传学是一个遗传学.
进行比较基因组学.

背景情况:

性别决定机制在动物分类中表现出显著的差异,甚至在物种内部.
像AB和TU这样的化斑马鱼 (Danio rerio) 菌株缺乏强大的遗传性决定位点.
野生来源的纳迪亚 (NA) 斑马鱼具有ZZ男性/ZW女性染色体性别确定系统.

研究的目的:

阐明纳迪亚 (NA) 斑马鱼菌株发生性腺发育的机制.
用组织学和单细胞转录学来比较ZZ和ZW NA斑马鱼的性腺发育.

主要方法:

在ZZ和ZW NA斑马鱼中发展性腺体的组织学分析.
在受精后的19日和30日进行性腺单细胞RNA测序 (scRNA-seq).
进行比较性转录基因分析以识别细胞类型和基因表达模式.

主要成果:

ZW NA斑马鱼在22dpf的时间内形成了卵细胞,而ZZ NA斑马鱼则直接形成了丸.
在19dpf的scRNA-seq显示了ZZ和ZW胚胎中类似的双潜性淋巴细胞类型.
在30dpf的scRNA-seq显示分别在ZZ和ZW生殖腺中存在明显的丸 (Sertoli,Leydig) 和卵巢 (granulosa,theca) 细胞转录组.

结论:

年轻的NA斑马鱼最初会发展出双能性生殖腺.
在NA W染色体上的一个因素,或两个Z染色体的缺失,对于启动卵细胞发育至关重要.
AB和TU斑马鱼菌株在化过程中可能丢失了Z染色体,模仿了WW斑马鱼,并解释了它们改变的性别决定.

关键词:

双潜性生殖腺 (BIPOTENTIAL GONAD) 是一种双潜性生殖腺.发生性腺发育的发展.造血细胞是造血细胞的组成部分.间歇性/流体细胞.青少年卵巢青少年卵巢这就是 scRNA-seqq.性别的确定性性别的确定性单细胞转录学转录学

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