碳点的调节过氧化酶类活性,用于对酸酸酶的色度检测
Yongmei Zhang1, Haibo Liang1, Xinru Wang1
1Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou, Guangdong, China.
Applied spectroscopy
|March 26, 2024
概括
添加碳点 (P-CDs) 提供可调节的过氧化酶类活性,用于检测酸酸酶 (ACP). 这种新的纳米酶方法使得在人类血清样本中能够进行敏感和选择性的ACP量化.
科学领域:
- 生物化学 生物化学
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 精确调节纳米酶活性对于生物感知至关重要.
- 碳点 (CD) 显示出有前途的过氧化酶类活性.
- 开发针对酸酸酶 (ACP) 的选择性生物传感器非常重要.
研究的目的:
- 开发一种敏感和有选择的方法,用于使用添加碳点 (P-CDs) 来检测ACP.
- 调节P-CDs的类似过氧酶的活性,以提高生物感知性能.
- 为ACP建立一个成本效益高的色度检测技术.
主要方法:
- 通过一热水法合成添加碳点 (P-CDs).
- 调节的P-CDs通过控制植物酸前体度来控制催化活性.
- 开发了一种色度测定方法,将P-CDs纳米酶和L-ascorbyl-2-phosphate三盐 (AAP) 的ACP催化水解相结合.
主要成果:
- P-CDs表现出可调节的过氧化酶类活性,催化TMB氧化.
- 通过将AAP化为甲酸 (AA),ACP抑制了P-CDs的活性.
- 试验显示,ACP的线性反应范围为0.4-4.0 mU/mL,检测极限为0.12 mU/mL.
- 在人血清样本中成功应用,回收率为98.7-101.6%.
结论:
- 兴奋剂有效调节用于生物感知的纳米酶活性.
- 开发的基于P-CD的色度测量方法提供了灵敏和选择性的ACP检测.
- 这种方法为基于纳米酶的生物传感应用提供了低成本和有效的策略.
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