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Updated: Jun 29, 2025

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对水牛介质蛋白-17A的分子和结构洞察

Savita Budania¹, Surinder Kumar Kadian¹, Karuppiah Kanagarajadurai²

¹Department of Veterinary Microbiology and Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, Haryana, India.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

|March 26, 2024

概括

研究人员克隆并分析了水牛的IL-17A基因,发现它与牛IL-17A非常相似. 这种重组水牛IL-17A蛋白显示出作为诊断和治疗的免疫生物试剂的潜力.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

介素-17A (IL-17A) 是一种关键的促炎性细胞因子,参与免疫反应对病原体和自身免疫性疾病.
此外,IL-17A还可以保护胃肠道和呼吸道粘膜的细菌和真菌感染.
对于水牛IL-17A的分子数据有限,尽管水牛在牛奶生产中的重要性.

研究的目的:

克隆,表达和分析印度穆拉水牛IL-17A基因.
以计算方式调查水牛IL-17A (buIL-17A) 的序列,结构和进化关系.
评估重组buIL-17A蛋白作为免疫生物试剂的潜力.

主要方法:

将水牛IL-17A基因克隆成一个核细胞表达载体 (pET-28a).
重组水牛IL-17A蛋白的表达,净化和表征.
对buIL-17A序列,结构和进化背景的生物信息分析.

主要成果:

没有信号的buIL-17A的编码序列长132个氨基酸,类似于牛.
雄牛和牛IL-17A之间的序列一致性为99%,只有一种氨基酸替代.
他们成功地表达和净化了复合的buIL-17A蛋白.

结论:

与牛相比,克隆和表达的水牛IL-17A蛋白质的保存率很高.
再组合的buIL-17A蛋白具有作为一种有价值的免疫生物学试剂的潜力.
这项研究提供了水牛IL-17A的基础分子数据,适用于诊断和治疗.

关键词:

它们的IL-17A是IL-17A.自身免疫性疾病自身免疫性疾病布法罗水牛是什么意思细胞因子细胞因子

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