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在小麦真菌病原体Bipolaris sorokiniana中发现了一种新的ToxA哈普洛型

Pao Theen See¹, Elyce M Iagallo¹, Kalai A Marathamuthu¹

¹Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia.

|March 26, 2024

概括

在澳大利亚Bipolaris sorokiniana分离物中发现了致死因效应因子ToxA,这是一种小麦病原体毒性因子. 新的ToxA变种表现出诱导死亡的活性,ToxA生成的分离物对小麦品种表现出多样化的毒性.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
菌类遗传学菌类遗传学

背景情况:

死亡效应因子ToxA是影响小麦的真菌病原体的关键毒性因子.
ToxA最初在Pyrenophora tritici-repentis中被发现,后来在Parastagonospora物种中被发现.
在小麦斑点的原因Bipolaris sorokiniana中,ToxA的存在和作用较少被探索.

研究的目的:

评估澳大利亚双极虫 (Bipolaris sorokiniana) 隔离物中ToxA的存在和多样性.
调查不同ToxA变异的功能活性和毒性贡献.
了解ToxA与携带Tsn1敏感性基因的小麦品种之间的相互作用.

主要方法:

使用分子方法选五个澳大利亚B. sorokiniana分离物以检测ToxA的存在.
确定ToxA单元型的测序和表征,包括通过大肠杆菌异质表达的功能分析.
对澳大利亚小麦品种的病毒性测定,比较具有不同ToxA配置文件和对ToxA (Tsn1) 敏感性的分离物.

主要成果:

在五个澳大利亚B. sorokiniana分离物中,有四个含有ToxA.
确定了两种ToxA单元型:已知的ToxA19和一种新型变体ToxA25,在168位发生了糖氨酸到氨酸突变.
两种B. sorokiniana ToxA变种都在对ToxA敏感的Tsn1品种中诱导了亡,而ToxA生成的分离物通常显示出更高的毒性.

结论:

澳大利亚B. sorokiniana种群表现出多样化的毒性,其中ToxA扮演着重要的角色.
这种新型的ToxA25变体保留了诱导亡的活性,有助于病原体的攻击性.
托克斯A-Tsn1相互作用对疾病严重性的影响在隔离物之间有所不同,突出显示了复杂的宿主-病原体动态.

关键词:

这种植物叫做Cochliobolus sativusus.大麦大麦大麦大麦大麦大麦大麦大麦消亡效应因子 (necrotrophic effector) 是一种消亡效应因子.斑点斑点斑点斑点斑点斑点.太阳斑点太阳斑点.

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