氧气水平会影响空气-液体界面培养中的卵管表皮功能
Jianchao Huo1,2, Aleksandra Maria Mówińska1,2, Ali Necmi Eren1,3
1Department of Reproduction Biology, Leibniz-Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.
Histochemistry and cell biology
|March 26, 2024
概括
研究卵巢上皮细胞功能需要了解氧气水平. 超生理氧加速分化,但可能会改变体内组织的复制能力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 受精和早期胚胎发育发生在卵管中.
- 在体内研究具有挑战性;因此,卵巢微环境细胞培养模型至关重要.
- 空气-液体接口 (ALI) 培养产生高度差异化的卵管上皮细胞.
研究的目的:
- 为了研究氧气水平对卵管表皮功能的影响,在体外.
- 为了比较使用ALI培养在生理 (5%) 和超生理 (18%) 氧气水平培养的猪卵管上皮细胞 (POEC).
- 评估氧气张力和介质对上皮质结构,屏障功能和分泌的影响.
主要方法:
- 在空气-液体界面 (ALI) 培养的猪卵管上皮细胞 (POEC).
- 使用了生理 (5%) 和超生理 (18%) 氧气张力.
- 评估了表皮结构,屏障功能,卵管液代用物 (OFS) 分泌和基因表达.
主要成果:
- 在所有条件下,ALI-POEC形成了极化,状单层,具有适当的屏障功能.
- 18%的氧气加速了分化,显著增加了顶端OFS体积和蛋白质含量.
- 卵管基因表达和OFS中的OVGP1丰度受氧张力和氧气介质的影响.
结论:
- 卵管上皮细胞适应体外超生理氧水平.
- 这种适应可能会影响细胞在体内复制组织特征的能力.
- 氧气张力是影响卵巢上皮细胞功能和培养中的分化的一个关键因素.
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