来自Tolypocladium inflatum的多重 prenyltransferase 的生物化学表征
Haiyan Han1, Shuang Peng1, Qian Wang1
1Key Laboratory for Enzyme and Enzyme-Like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, No. 26 Hexing Road, Harbin, 150040, Heilongjiang, China.
Applied microbiology and biotechnology
|March 26, 2024
概括
这项研究描述了TolF,这是来自Tolypocladium inflatum的prenyltransferase,它修改了像帕克西林这样的天然产品. 这项研究揭示了用于开发新型生物合成策略和制药的prenylation反应的洞察力.
科学领域:
- 生物化学 生物化学
- 自然产品化学 自然产品化学
- 酶学 是一种酶学.
背景情况:
- 前化对于自然产品的多样性和生物活性至关重要.
- 印度醇二烯是一种具有多种生物活性的自然产品类.
- 了解prenyltransferases是释放这些化合物的生物合成潜力的关键.
研究的目的:
- 为了功能性地表征TolF,Tolypocladium inflatum中的一种先转移酶.
- 为了研究TolF和相关酶TerF的基质特异性和区域选择性.
- 探索真菌前转移酶在天然产品合成中的潜在应用.
主要方法:
- 在Aspergillus oryzae. tolF基因的异质表达.
- 用帕克西林及其衍生物进行养实验.
- 在体外酶试验中,使用纯化的重组TolF和TerF.
主要成果:
- 当TolF在A. oryzae中表达并食帕西林时,产生了20-和22-prenylpaxilline.
- 托尔F对帕克西林及其减少形式β-帕克西醇进行了先化.
- 托尔F和特尔F表现出类似的基质耐受性,前化帕克西林,β-帕克西醇和特尔醇I.
结论:
- 托尔F是一种多重前基转移酶,具有修改醇二烯的潜力.
- 这项研究增强了对印醇二二烯生物合成中的前化作用的理解.
- 菌前转移酶为新的生物合成策略和制药开发提供了机会.
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