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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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相关实验视频

Updated: Jun 29, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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用于高分辨率结构研究的冷电磁样本准备.

Liguo Wang1, Christina M Zimanyi2

  • 1Laboratory for BioMolecular Structure, Brookhaven National Laboratory, Upton, NY 11973, USA.

Acta crystallographica. Section F, Structural biology communications
|March 26, 2024
PubMed
概括
此摘要是机器生成的。

单粒子冷电子显微镜 (SPA cryo-EM) 是确定生物分子结构的强大工具. 本指南提供了关于样本准备的实用建议,以提高新SPA冷EM项目的成功率.

关键词:
冷电子显微镜的使用方法高分辨率的结构确定.一个粒子分析分析.样本的准备 样本的准备

更多相关视频

Optimizing Sample Preparation for Cryogenic Electron Microscopy
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Optimizing Sample Preparation for Cryogenic Electron Microscopy

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Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

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相关实验视频

Last Updated: Jun 29, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

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Optimizing Sample Preparation for Cryogenic Electron Microscopy
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Optimizing Sample Preparation for Cryogenic Electron Microscopy

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Preparation of High-Temperature Sample Grids for Cryo-EM
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Preparation of High-Temperature Sample Grids for Cryo-EM

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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 单粒子冷电子显微镜 (SPA cryo-EM) 越来越多地用于高分辨率的生物分子结构确定.
  • 越来越多的人采用SPA冷-EM,因此需要为新研究人员提供可访问的指导.

研究的目的:

  • 在SPA冷EM中提供实用策略和建议,用于样品制备.
  • 要突出在SPA冷-EM样本准备过程中遇到的常见挑战和陷.
  • 为了提高新的SPA冷EM项目的成功率.

主要方法:

  • 对SPA冷EM的常见样品制备技术的审查.
  • 确定影响冷EM的样本质量的关键因素.
  • 讨论潜在的陷和故障排除策略.

主要成果:

  • 概述了成功准备SPA冷-EM样本的重要考虑因素.
  • 确定优化样本行为和数据质量的关键策略.
  • 提供实用技巧,以在工作流程中应对常见的挑战.

结论:

  • 有效的样本准备对于成功的SPA冷-EM结构确定至关重要.
  • 了解技术的局限性和采用最佳实践可以提高项目成功率.
  • 本指南旨在使研究人员有能力自信地开始SPA冷EM研究.