一个OMT集群的新功能化主导着与类植物化相关的聚甲基黄生物合成
Zhaoxin Peng1,2, Lizhi Song1, Minghua Chen1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
概括
三种类O-甲基转移酶基因 (OMTs) 是生产聚甲基 (PMFs) 的关键,聚甲基 (PMFs) 以抗癌特性而闻名. 基因重复和促进体变化解释了普通中PMF的积累,有助于未来的繁殖以获得健康益处.
科学领域:
- 植物生物化学 植物生物化学
- 代谢学 代谢学 代谢学
- 遗传学 是一个遗传学.
背景情况:
- 聚甲类黄 (PMFs) 是类植物中具有显著抗癌潜力的专门代谢物.
- 负责将基黄素广泛O-甲基化为PMF的特定O-甲基转移酶 (OMT) 基本上尚未确定.
- 在普通中大量存在PMF,但在较早的类品种中却不存在.
研究的目的:
- 为了确定参与类植物PMF生物合成的特定OMT基因.
- 阐明在普通中PMF积累背后的进化机制.
- 了解类物种内PMF含量变化的遗传基础.
主要方法:
- 对比基因组学和合成分析以确定候选OMT基因.
- 在体外和体外酶分析以确定OMT对氧黄的活性.
- 对基因重复事件和促进区域进行分析,以获得进化见解.
主要成果:
- 三个同源基因CreOMT3,CreOMT4和CreOMT5被确定为PMF生物合成的关键.
- 这些OMT显示了多位点O-甲基化活性,产生了七种不同的PMF.
- 对比分析表明,从CreOMT6中对OMT基因 (CreOMT3-5) 的协同重复,通过新功能化促进了普通的PMF路径演变.
- 在CreOMT4中,一种特定的氨基酸残留物 (N17) 对于其甲基化活性至关重要.
- 现代种植 mandarins 的 CreOMT4 促进体中的 1,041-bp 删除与 PMF 水平的降低有关.
结论:
- CreOMT3,CreOMT4和CreOMT5是主要的OMT,负责果中的PMF生产.
- 基因重复和OMT的新功能化在普通话血统中PMF生物合成的进化中发挥了重要作用.
- 关键OMT基因中的促进子变异,如CreOMT4,有助于在种植的类品种中观察到的PMF积累的定量差异.
- 这项研究为了解PMF途径和开发含有增强健康促进化合物的类植物提供了基础.
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