Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Nucleosome Remodeling02:54

Nucleosome Remodeling

9.1K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.1K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.5K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.5K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

12.1K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
12.1K
The Nucleosome01:19

The Nucleosome

1.5K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
1.5K
Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

DNA Sequence and Histone Variant H2A.Z Jointly Govern Nucleosome Unwrapping Pathways.

bioRxiv : the preprint server for biology·2026
Same author

Structural dynamics in the CENP-A nucleosome impacted by protein-protein interactions with centromere protein N.

Nanoscale·2026
Same author

H2A.Z facilitates Sox2-nucleosome interaction by promoting DNA and histone H3 tail mobility.

Nucleic acids research·2026
Same author

Mapping Allosteric Communication in the Nucleosome with Conditional Activity.

Journal of chemical information and modeling·2026
Same author

From Molecules to Mechanisms: Integrating MD and Stochastic Modeling to Decipher RXR-RAR Gene Regulation.

The journal of physical chemistry. B·2025
Same author

Mapping Allosteric Communication in the Nucleosome with Conditional Activity.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Jun 29, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.1K

核动力学中的序列依赖性

Prabir Khatua1, Phu K Tang1,2, Abhik Ghosh Moulick1

  • 1Department of Chemistry, College of Staten Island, The City University of New York, 2800 Victory Boulevard, Staten Island, New York 10314, United States.

The journal of physical chemistry. B
|March 26, 2024
PubMed
概括
此摘要是机器生成的。

DNA 序列影响核细胞的定位和动态. 分子动力学模拟揭示了特定序列的DNA解路径和基因尾相互作用,影响色素结构.

更多相关视频

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

11.6K
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

1.8K

相关实验视频

Last Updated: Jun 29, 2025

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.1K
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

11.6K
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

1.8K

科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.
  • 计算生物学 计算生物学

背景情况:

  • 核细胞是真核染色体的基本单元,由DNA包裹在基因组蛋白质周围组成.
  • 众所周知,DNA序列会影响核细胞的定位,但控制这种相互作用和核细胞动态的分子机制尚未完全理解.

研究的目的:

  • 在分子层面上研究DNA序列和核酶体动力学之间的相互作用.
  • 通过全原子分子动力学模拟,阐明依赖序列的DNA解路径.

主要方法:

  • 用人类α卫星平行模型 (ASP) 和Widom-601 DNA序列对核体进行了全原子分子动力学模拟.
  • 在高盐度 (10-20倍生理) 中模拟核体以选静电相互作用并促进DNA解封.
  • 分析了微秒时间尺度的模拟,以了解依赖于序列的DNA解封事件.

主要成果:

  • ASP DNA 序列在 ±5 的超螺旋位置周围促进了循环形成,与增加的基因素尾部接触和对释放有关.
  • 维多姆-601DNA序列在DNA末端表现出显著的呼吸运动,与N端H3尾部崩和α螺旋形成有关.
  • 在DNA解封和基因尾相互作用中观察到明显的依赖序列的行为.

结论:

  • DNA 序列关键地决定了核体动力学和 DNA 解封路径.
  • 基因组尾动力学和潜在的翻译后修饰 (PTMs) 可能是核酶体动力学的关键调节者.
  • 这些发现为特定序列的染色质组织和调节提供了分子洞察力.