相关实验视频
Updated: Jun 29, 2025
07:55
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
Published on: June 26, 2020
10.2K
H3K4me1在植物中招募DNA修复蛋白
Daniela Quiroz1,2, Satoyo Oya1,3, Diego Lopez-Mateos4,5
1Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.
The Plant cell
|March 26, 2024
概括
在植物中,DNA修复蛋白 MUTS HOMOLOG 6 (MSH6) 和 PRECOCIOUS DISSOCIATION OF SISTERS 5 (PDS5C) 与 H3K4me1 结合,从而降低了基因丰富地区的突变率. 这种表观基因组修复机制在植物物种中得到保护.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- DNA 修复蛋白利用基因组读取器域来准特定的表观基因组特征.
- 基因组基因组基因组内突变率的变化受到基因组内基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组基因组.
- H3K4me1是一种与基因体和基本基因相关联的基因标记.
研究的目的:
- 在植物中研究H3K4me1相关的低突变机制.
- 检查 MUTS HOMOLOG 6 (MSH6) 和 PRECOCIOUS DISSOCIATION OF SISTERS 5 (PDS5C) 在 H3K4me1 中介的 DNA 修复中的作用.
- 为了确定这些机制在植物物种中的保护.
主要方法:
- 研究了Arabidopsis thaliana和Oryza sativa (大米) 的植物.
- 利用超深度测序来分析野生类型和msh6淘汰赛线的突变率.
- 研究了MSH6和PDS5CTudor域与H3K4me1.1的结合.
- 检查了一大批大米突变数据集.
主要成果:
- 在Arabidopsis中的MSH6Tudor域与H3K4me1.1结合.
- 功能性MSH6对于减少H3K4me1丰富基因体中的单基替代 (SBS) 突变至关重要.
- 与H3K4me1相关的低基突变和MSH6和PDS5C的H3K4me1结合残留物被保留在米中.
- 植物表观基因组招募的DNA修复机制是融合的,但与人类机制不同.
结论:
- H3K4me1招募DNA修复蛋白,包括MSH6和PDS5C,到植物的特定基因组区域,从而降低突变率.
- 这种表观基因组修复系统在植物中得到保存,并为突变速率的变化提供了机械洞察力.
- 这些发现揭示了由表观基因组特征介导的独特的植物特异性DNA修复策略.
相关概念视频
02:31Homologous Recombination
50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
01:38Nucleotide Excision Repair
3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
02:37DNA Damage can Stall the Cell Cycle
9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
01:02Long-patch Base Excision Repair
7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
01:54Base Excision Repair
22.3K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
22.3K
01:20Mismatch Repair
4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K