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在常见小麦麦中,肠诱导,其次是再生和毛状根诱导

Denis O Omelchenko¹, Elena S Glagoleva^1,2, Anna Y Stepanova³

¹Institute for Information Transmission Problems of the Russian Academy of Sciences, Moscow, Russia.

Methods in molecular biology (Clifton, N.J.)

|March 27, 2024

概括

使用Agrobacterium rhizogenes开发了麦 (Fagopyrum esculentum) 植物再生和转基因毛发根诱导的优化协议. 这些方法提高了基因修饰和在不同品种的繁殖效率.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
农业科学农业科学

背景情况:

感应和植物再生对于麦 (Fagopyrum esculentum) 的生物技术应用至关重要.
通过Agrobacterium rhizogenes介导的毛发根诱导对于功能性基因研究和基因组修饰至关重要.
现有的协议显示不同麦品种的效率可变.

研究的目的:

为了优化诱导,植物再生,和麦的Agrobacterium rhizogenes介导的转化协议.
评估跨多种麦品种的协议效率,包括野生亲属.
为了确定劳动有效的感染方法,以成功启动毛发根.

主要方法:

优化了Murashige-Skoog (MS) 基底介质,具有特定的激素度,用于诱导,射击启动,繁殖和根启动.
使用Agrobacterium rhizogenes菌株R1000用于毛发根诱导和T-DNA转移.
雇佣的苗木根部浸泡和带刺用于Agrobacterium感染.

主要成果:

为麦植物再生和转型的每个阶段建立了最佳介质配方.
在诱导毛发根和T-DNA转移方面,Agrobacterium rhizogenes R1000表现出高效率.
劳动有效的感染方法实现了100%的毛发根生长启动在扩散物中.

结论:

开发了高效和优化的协议,用于麦植物再生和转基因毛发根培养.
优化的方法适用于各种麦品种,包括野生祖先.
这项研究为麦遗传改进和功能基因组学研究提供了宝贵的工具.

关键词:

通过农业细菌介导的转化过程.卡勒斯 (Callus) 是一个叫做卡勒斯的人.这种植物是Fagopyrum esculentum.有毛的根,毛的根.植物再生植物再生

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