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Updated: May 6, 2026

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
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用Confocal显微镜,ImageJ和R Studio进行Fagopyrum核表观遗传修饰的定量分析

Alicja Tomasiak1, Lea Sophie Berg2, Katarzyna Sala1

  • 1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.

Methods in molecular biology (Clifton, N.J.)
|March 27, 2024
PubMed
概括

本研究详细介绍了一种分析Fagopyrum calli表观遗传修饰的方法,该方法使用免疫染和共聚焦显微镜. 该协议通过检查基因组和DNA甲基化来帮助理解发育期间的基因调节.

关键词:
同焦点显微镜的共聚焦显微镜表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.工作室 R 工作室 R 工作室

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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