再组合蛋白酶生产的进展:现状和前景
Xiufang Liu1,2, Mulin Lian1,2, Mouming Zhao1,2
1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
World journal of microbiology & biotechnology
|March 27, 2024
概括
重组蛋白酶的生产对于满足工业需求至关重要. 本综述探讨了微生物表达系统和工程技术,以提高蛋白酶产量和应用兼容性.
科学领域:
- 生物化学和分子生物学
- 微生物生物技术 微生物生物技术
背景情况:
- 蛋白酶是重要的酶,在食品,生物技术和医学中广泛应用.
- 原生蛋白酶宿主通常限制高水平表达和下游处理.
- 再组合生产为克服这些局限性提供了一个解决方案.
研究的目的:
- 审查微生物系统中蛋白酶生产的现状.
- 为了突出过去五年来蛋白酶工程的进步.
- 提供对重组蛋白酶研究未来的见解.
主要方法:
- 关于 prokaryotic (Bacillus spp.) 的概况 和真核生物 (酵母) 表达系统用于蛋白酶生产.
- 讨论最近的蛋白酶工程策略,包括理性设计和定向进化.
- 综合当前趋势和重组蛋白酶研究中的关键发现.
主要成果:
- 细菌物种是蛋白酶表达的主要原生体宿主.
- 酵母被广泛用作真核体表达系统.
- 蛋白酶工程通过理性设计和定向进化取得了重大进展.
结论:
- 微生物系统中的重组蛋白酶生产对于工业需求至关重要.
- 表达系统和工程技术的进步正在推动进步.
- 未来的研究有望进一步优化蛋白酶的生产和应用.
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