在T2DM及其相关的微血管糖尿病并发症中的基因表达分析:关注风险因素和RAAS途径
Laxmipriya Jena1, Prabhsimran Kaur1, Tashvinder Singh1
1Department of Human Genetics and Molecular Medicine, Central University of Punjab, Bathinda, 151401, India.
Molecular neurobiology
|March 27, 2024
概括
2型糖尿病 (T2DM) 的并发症与氨酸氨酸多素系统 (RAAS) 基因的改变表达有关. 高BMI和吸烟等危险因素也会导致T2DM及其微血管并发症.
科学领域:
- 内分泌学和新陈代谢学
- 遗传学和分子生物学
背景情况:
- 在2型糖尿病 (T2DM) 中长时间的高血糖导致影响脏,视网膜和神经的微血管并发症.
- 氨酸- ангиотензин- алдостерон系统 (RAAS) 与胰岛素耐药性和T2DM相关的并发症有关.
研究的目的:
- 研究T2DM患者风险因素与RAAS基因表达之间的关联.
- 将T2DM患者的RAAS基因表达与没有糖尿病并发症的患者与健康对照进行比较.
主要方法:
- 一项涉及420名受试者的横截面研究:140名健康对照,140名患有并发症的T2DM患者和140名没有并发症的T2DM患者.
- 对T2DM及其并发症的风险因素的评估.
- 定量实时PCR (qRTPCR) 用于分析RAAS基因 (AGT,ACE,ACE2,AGT1R) 的表达.
主要成果:
- 已确定T2DM和并发症的风险因素包括城市生活,高BMI,酒,吸烟和家族史.
- 与对照组相比,在T2DM患者 (有并发症和没有并发症) 中观察到AGT,ACE和AGT1R基因表达的升调.
- 与对照组相比,在T2DM患者 (有并发症和没有并发症) 中发现了ACE2基因表达的下调.
结论:
- 改变RAAS通路基因的表达与T2DM中微血管糖尿病并发症的发展有关.
- 特定的RAAS基因表达模式可能作为糖尿病并发症的指标或贡献者.
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