使用Acidipropionibacteriumacidipropionici,从葡萄糖和甘油/葡萄糖混合物中增强酸生产的高细胞密度连续批次发酵
Tarek Dishisha1, Mridul Jain2, Rajni Hatti-Kaul3
1Department of Pharmaceutical Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni Suef, 62511, Egypt.
Microbial cell factories
|March 27, 2024
概括
高细胞密度发酵与细胞循环增强了从葡萄糖和原始甘油中产生酸 (PA). 这种微生物路线利用工业副产品来提高可行性.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 代谢工程是代谢工程.
背景情况:
- 来自可再生资源的酸 (PA) 发酵在体积生产率和产品度方面面临挑战,阻碍了工业应用.
- 高细胞密度 (HCD) 发酵技术为克服这些局限性提供了解决方案.
- 利用农业工业副产品作为营养来源对于经济可行性至关重要.
研究的目的:
- 为了评估由Acidipropionibacterium acidipropionici使用HCD批发发酵与细胞回收进行酸 (PA) 生产.
- 评估使用葡萄糖和原始甘油/葡萄糖混合物作为碳来源的可行性.
- 研究热处理的土豆汁 (HTPJ) 作为源的使用.
主要方法:
- 使用连续细胞循环的HCD批发发酵.
- 发酵是使用葡萄糖或原始甘油/葡萄糖混合物进行的.
- 用热处理的土豆汁 (HTPJ) 作为源.
主要成果:
- 40g/L葡萄糖的发酵平均每批产生18.76g/LPA,最大速率为1.15g/L/h,生物质为39.89gCDW/L.
- 粗糖/葡萄糖 (60g/L:30g/L) 的共同发酵,以0.35g/L/h的速度平均每批产生35.36g/LPA,达到12.66gCDW/L生物质.
- 生物补充剂增强了细胞生长和PA生产力,但增加了酸副产品的形成.
结论:
- 用细胞回收利用的HCD批量发酵是工业酸生产的一个有前途的战略.
- 使用工业副产品共同发酵原油甘油和葡萄糖,实现了报告的高生产率.
- 这种方法提高了微生物酸生产的工业可行性.
更多相关视频
09:28Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Published on: May 18, 2020
8.4K
06:45Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
Published on: August 9, 2024
1.1K
相关概念视频
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
