父亲的衰老影响表达和表观遗传标记,早在第一个胚胎组织谱系分化早期
Michelle M Denomme1, Blair R McCallie1, Mary E Haywood1
1CCRM Genetics, 10290 Ridgegate Circle, Lone Tree, CO, 80124, USA.
Human genomics
|March 27, 2024
概括
先进的父亲年龄与后代神经发育障碍的风险更高有关. 这项研究发现胚胎发育早期的表观遗传和基因表达变化,影响未来的胎儿健康.
科学领域:
- 生殖生物学和发育表观遗传学.
- 人类胚胎学和植入前发展.
- 在疾病风险中的基因组学和转录组学.
背景情况:
- 先进的父亲年龄 (APA) 与不良后代健康结果有关,包括神经发育障碍.
- 与父亲年龄相关的早期胚胎血统 (ICM和TE) 的甲基组和转录组的研究.
- 利用捐赠卵细胞的试管婴儿周期来控制母亲年龄的影响.
研究的目的:
- 研究与父亲年龄相关的人类胚胎细胞系 (ICM和TE) 的甲基组和转录组.
- 确定与晚年父亲年龄相关的早期胚胎发育中的分子变化.
- 探索这些改变对后代神经发育障碍风险的潜在影响.
主要方法:
- 从IVF周期中采集人体胚胎囊,其父的年龄较大 (≥50岁) 或年轻的父亲.
- 机械分离芽细胞囊成内部细胞质量 (ICM) 和皮 (TE) 样本.
- 在分离的ICM和TE样本上进行甲基组和转录组分析.
主要成果:
- 在ICM和TE中观察到显著的并发差异甲基化和转录,来自APA衍生芽细胞.
- 甲基组分析显示,神经元信号通路的丰富和神经发育障碍的关联在两种谱系中都有.
- 转录组分析显示ICM中神经发育信号通路的丰富,但TE中没有.
结论:
- 父亲的衰老导致早期胚胎血统中的甲基化和转录失调.
- 植入前胚胎中的这些分子变化与后代神经发育障碍的风险增加有关.
- 来自APA父亲的胚胎没有初始植入妥协,但早期的表观遗传标记表明未来的胎儿易感性.
关键词:
晚年父亲的年龄.胚胎囊中的胚胎囊.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.细胞内部质量 细胞内部质量甲基组组是指甲基组组中的一种.神经发育障碍 神经发育障碍神经元信号通道的神经元信号通道后代的健康后代的健康转录组 转录组就是一个转录组.托菲克多德的皮肤.更多相关视频
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