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一个转录基因数据集,用于研究在慢性,蛋白毒性内分泌网膜应激状态下的阿拉比多普西斯未折叠蛋白质反应

Amélie Ducloy¹, Marianne Azzopardi¹, Caroline Ivsic^1,2

¹University Paris-Saclay, INRAE, AgroParisTech, Institute Jean-Pierre Bourgin (IJPB), 78000 Versailles, France.

|March 27, 2024

概括

在Arabidopsis thaliana中,未折叠的蛋白质反应 (UPR) 被尼胺激活,对野生型和IRE1缺乏突变的基因表达有不同的影响. 这一数据集揭示了器官特异性的转录基因变化,这些变化对于理解ER压力和植物生理学至关重要.

科学领域:

植物分子生物学植物分子生物学
细胞应激反应细胞应激反应
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

展开的蛋白质响应 (UPR) 是一个保存的信号通路,调节内 плазма网膜 (ER) 恒常性.
在植物中,UPR对于发育,繁殖,免疫力和耐压力至关重要.
ER压力,由诸如图尼卡米辛 (Tm) 等药物诱导,激活了UPR.

研究的目的:

在ER压力下的Arabidopsis thaliana幼苗的转录组景观的特征.
为了区分UPR依赖和UPR独立的基因表达变化.
为了研究在芽和根中对ER压力的器官特异反应.

主要方法:

进行RNA测序 (RNA-seq) 在14天大的Arabidopsis thaliana幼苗上进行.
种子包括野生类型 (WT) 和缺少IRE1A和IRE1B的双突变.
图尼卡米辛 (Tm) 治疗用于诱导ER压力;分别分析了芽和根.

主要成果:

图尼卡米辛诱导了在WT和突变幼苗中数百个差异表达基因 (DEGs).
在拍摄过程中,与在Tm压力下的WT相比,在双变异体中,分别有872和563个基因的上调和下调.
在根基中,425和439个基因在双重突变中分别显著上调和下调,与在Tm压力下的WT相比.

结论:

这项研究提供了一个全面的转录基因数据集,用于Arabidopsis.ER的应激反应.
这些数据允许识别由IRE1依赖和IRE1独立通路调节的基因.
这一数据集对于未来对ER恒温和植物应激生理学的研究有价值.

关键词:

与阿斯帕拉金相关的糖基化.需要伊诺西的-1-1酶.植物植物植物植物植物.蛋白质的平衡是蛋白质的平衡.根系根系根系根系射击射击射击尼卡米辛是一种尼卡米辛.

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