从印度尼西亚人口中分离出的Streptococcus pneumoniae的全基因组测序数据
Miftahuddin Majid Khoeri1,2, Yustinus Maladan1, Korrie Salsabila1
1Eijkman Research Center for Molecular Biology, National Research and Innovation Agency, Cibinong, West Java, Indonesia.
Data in brief
|March 27, 2024
概括
来自印度尼西亚的94个Streptococcus pneumoniae分离物的全基因组测序为分析肺炎球菌合疫苗 (PCV) 影响提供了基线. 这些数据对于了解抗菌素耐药性和毒性因素至关重要.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 疫苗学 疫苗学 疫苗学
背景情况:
- 肺炎菌是全球细菌性肺炎,细菌病和脑膜炎的主要原因.
- 印度尼西亚在2022年实施了一项全国性肺炎球菌合疫苗 (PCV) 计划.
研究的目的:
- 提出在PCV计划之前在印度尼西亚收集的S. pneumoniae分离物的全基因组序列 (WGS) 数据.
- 为未来关于疫苗影响,抗菌素耐药性和毒性研究建立基因组基线.
主要方法:
- 使用Illumina NovaSeq6000对94个S. pneumoniae分离体进行全基因组测序.
- 使用TruSeq纳米DNA套件进行DNA提取.
主要成果:
- 染色体大小 (1.97-2.74 Mbp) 和G+C含量 (39-40%) 的基因组数据.
- 每个基因组确定了1935-3319编码序列 (CDS),2-5rRNA,43-49tRNA和56-71ncRNA.
- 生成的公开可用的WGS数据 (BioProject PRJNA995903).
结论:
- 世界地质系统数据为印度尼西亚的公共卫生倡议提供了基础资源.
- 能够详细分析血清型,序列类型和病毒性和抗生素耐药性的遗传决定因素.
- 方便监测PCV计划对S. pneumoniae种群的长期影响.
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